語言

Chang, Chi-Fon

CFChang6x7Senior Research Specialist/Scientist

chifongateEmail
+886-2-27871236

 

EDUCATION AND POSITIONS HELD:

  • Ph.D. , Biophysics, The Ohio State University ,1997
  • Postdoctoral Fellow, Institute of Biomedical Sciences, Academia Sinica , 1997-1999
  • NMR Supporting Team Manager, Institute of Biomedical Sciences, Academia Sinica,1999-2002
  • NMR Facility Manager, High-Field Biomacromolecular Solution NMR Core Facility, Academia Sinica ,2002-present
  • Associate Research Specialist/Scientist, Genomics Research Center, Academia Sinica, 2004-2010
  • Senior Research Specialist/Scientist, Genomics Research Center, Academia Sinica, 2010-present

FACILITY MANAGEMENT / EXPERTISE:

As a research specialist, I am responsible for the daily operation on NMR facility and assist researchers carrying out NMR related researches. I have dedicated myself to provide competent and efficient routine service, as well as all aspects of advance technical support on NMR spectroscopy. The duty of my job includes: keeping track of machine time usage; supervising the facility staff members; training users on using NMR spectrometers; developing and implementing new NMR experiments; assisting users setting up advanced NMR experiments; advising users on specific NMR experiments and applications; and providing technical consultation. My expertise is using biophysical methods, especially the NMR approach, to obtain structural and functional information for biomolecules.

 NMR Facility
 GRC NMR Facility

SELECTED PUBLICATIONS:

  • Lin MH, Huang YP, Chang CF*, Hsu CH*, 2021, “NMR assignments of the macro domain from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).”, Biomolecular NMR assignments, 15(1), 137-142. (SCIE)
  • Lin MH, Cho CC, Chiu YC, Chien CY, Huang YP, Chang CF, Hsu CH, 2021, “Elucidating the tunability of binding behavior for the MERS-CoV macro domain with NAD metabolites.”, Communications biology, 4(1), 123. (SCIE)
  • Mariasina SS, Chang CF, Petrova OA, Efimov SV, Klochkov VV, Kechko OI, Mitkevich VA, Sergiev PV, Dontsova OA, Polshakov VI, 2020, “Williams-Beuren syndrome-related methyltransferase WBSCR27: cofactor binding and cleavage.”, The FEBS journal, 287(24), 5375-5393. (SCIE)
  • Mariasina SS, Petrova OA, Osterman IA, Sergeeva OV, Efimov SV, Klochkov VV, Sergiev PV, Dontsova OA, Huang TH, Chang CF*, Polshakov VI*, 2018, “NMR assignments of the WBSCR27 protein related to Williams-Beuren syndrome.”, Biomolecular NMR assignments, 12(2), 303-308. (SCIE)
  • Lin Tien-Wei, Chang Chi-Fon, Chang Yu-Jen, Liao Yi-Hung, Yu Hui-Ming, Chen Yun-Ru, 2017, “Alzheimer’s amyloid-β A2T variant and its N-terminal peptides inhibit amyloid-β fibrillization and rescue the induced cytotoxicity”, PLOS ONE, 12(3), e0174561. (SCIE)
  • Chou CY, Chu M, Chang CF, Yu T, Huang TH, Sakellariou D, 2016, “High sensitivity high-resolution full range relaxometry using a fast mechanical sample shuttling device and a cryo-probe.”, Journal of biomolecular NMR, 66(3), 187-194. (SCIE)
  • Huang YP, Cho CC, Chang CF*, Hsu CH*, 2016, “NMR assignments of the macro domain from Middle East respiratory syndrome coronavirus (MERS-CoV).”, Biomolecular NMR assignments, 10(2), 245-248. (SCIE)
  • Sabater Laurent, Fang Pei-Ju, Chang Chi-Fon, De Rache Aurore, Prado Enora, Dejeu Jérôme, Garofalo Antonio, Lin Jung-Hsin, Mergny Jean-Louis, Defrancq Eric, Pratviel Geneviève, 2015, “Cobalt(iii)porphyrin to target G-quadruplex DNA”, Dalton Transactions, 44(8), 3701-3707. (SCIE)
  • Hsu CH, Chang CF, Liao YD, Wu SH, Chen C, 2015, “Solution structure and base specificity of cytotoxic RC-RNase 2 from Rana catesbeiana.”, Archives of biochemistry and biophysics, 584, 70-8. (SCIE)
  • Huang SY, Chang CF, Fang PJ, Naik MT, Guntert P, Shih HM, Huang TH*, 2015, “The RING domain of human promyelocytic leukemia protein (PML).”, Journal of biomolecular NMR, 61(2), 173-80. (SCIE)
  • Li YC, Chang CK, Chang CF, Cheng YH, Fang PJ, Yu T, Chen SC, Li YC, Hsiao CD, Huang TH, 2014, “Structural dynamics of the two-component response regulator RstA in recognition of promoter DNA element.”, Nucleic acids research, 42(13), 8777-88. (SCIE)
  • Huang SY, Naik MT, Chang CF, Fang PJ, Wang YH, Shih HM, Huang TH, 2014, “The B-box 1 dimer of human promyelocytic leukemia protein.”, Journal of biomolecular NMR, 60(4), 275-81. (SCIE)
  • Ming-Yuan Su, Chung-I Chang and Chi-Fon Chang* , 2013, “1H, 13C and 15N resonance assignments of the pyrin domain from human PYNOD ”, Biomolecular NMR Assignments, 7(2), 141-143. (SCIE)
  • Ming-Yuan Su, Chiao-I Kuo, Chi-Fon Chang*, Chung-I Chang*, 2013, “Three-Dimensional Structure of Human NLRP10/PYNOD Pyrin Domain Reveals a Homotypic Interaction Site Distinct from Its Mouse Homologue”, PLoS One, 8(7), e67843. (SCIE)
  • Ariesandi W, Chang CF, Chen TE, Chen YR*, 2013, “Temperature-dependent structural changes of Parkinson\'s alpha-synuclein reveal the role of pre-existing oligomers in alpha-synuclein fibrillization.”, PloS one, 8(1), e53487. (SCIE)