研究技師

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EDUCATION AND POSITIONS HELD:

• B.Eng, Chemical Engineering, National Tsing Hua University, Taiwan, 1993
• Ph.D., Life Sciences, National Tsing Hua University, Taiwan, 1999
• Post-doctoral Research Associate, Keck Graduate Institute of Applied Life Science, USA, 2000-2005
• Assistant Research scientist, Genomics Research Center, Academia Sinica, 2005-2008
• Associate Research scientist, Genomics Research Center, Academia Sinica, 2008-2012
• Senior research scientist, Genomics Research Center, Academia Sinica, 2012-present

EXPERTISE:

Development of biological assays for functional evaluation of therapeutically significant targets or molecules.

RESPONSIBILITY:

My main responsibilities towards GRC mission is to develop bioassay platforms for high-throughput screening, to conduct activity evaluations for lead identification/optimization, and to support the needs in assay development for researchers with main focuses on:

I. High-Throughput Screening

• Design and develop high-throughput compatible target- and cell-based assays
• Conduct high-throughput screening and hit confirmation
• Support further studies for lead identification/optimization

II. Assay Development

• Develop assays and evaluate inhibitor potencies for cell-wall synthesis enzymes to support efforts in discovery of small molecules against methicillin-resistant Staphylococcus aureus, Mycobacterium tuberculosis, and Gram-negative bacteria
• Support the needs for reagent preparation and for animal studies in vaccine as well as therapeutics evaluation for infectious diseases and cancer
• Evaluate the biological activities of fractions and small molecules derived from Chinese medicinal herbs to support the identification of active molecules for drug discovery

主要支援基因體中心研究團隊對活性分析的需求；例如針對有興趣的藥物標的建立生化分析及活體內活性分析系統並執行藥物篩選。目前的協助範圍包含了:

• 利用分生及生化技術發展藥物標的之生化活性偵測系統, 如酵素反應
• 設計藥物標的之活體內活性偵測系統來決定化合物在活體內之活性,
• 應用分析系統進行高速藥物篩選選擇具活性之化合物,
• 評估及測試小分子及天然化合物的生物活性, 以及
• 評估疫苗的效果等。

SELECTED PUBLICATIONS:

• Tseng YW, Yang TJ, Hsu YL, Liu JH, Tseng YC, Hsu TW, Lu Y, Pan SH,* Cheng TR,* Fang JM*, 2024, “Dual-targeting compounds possessing enhanced anticancer activity via microtubule disruption and histone deacetylase inhibition.”, European journal of medicinal chemistry, 265, 116042. (SCIE)
• Wang SC, Liao HY, Zhang JY, Cheng TR,* Wong CH*, 2019, “Development of a universal influenza vaccine using hemagglutinin stem protein produced from Pichia pastoris.”, Virology, 526, 125-137. (SCIE)
• Wu WS, Cheng WC, Cheng TR,* Wong CH*, 2018, “Affinity-Based Screen for Inhibitors of Bacterial Transglycosylase.”, Journal of the American Chemical Society, 140, 2752-2755. (SCIE)
• Huang LY, Wang SC, Cheng TR,* Wong CH*, 2017, “Undecaprenyl Phosphate Phosphatase Activity of Undecaprenol Kinase Regulates the Lipid Pool in Gram-Positive Bacteria.”, Biochemistry, 56(40), 5417-5427. (SCIE)
• Huang LY, Huang SH, Chang YC, Cheng WC, Cheng TJ, Wong CH, 2014, “Enzymatic synthesis of lipid II and analogues.”, Angewandte Chemie-International Edition, 53, 8060-8065. (SCIE)
• Huang SH, Wu WS, Huang LY, Huang WF, Fu WC, Chen PT, Fang JM, Cheng WC, Cheng TJ, Wong CH, 2013, “New continuous fluorometric assay for bacterial transglycosylase using forster resonance energy transfer.”, Journal of the American Chemical Society, 135(45), 17078-17089. (SCIE)
• Liao HY, Hsu CH, Wang SC, Liang CH, Yen HY, Su CY, Chen CH, Jan JT, Ren CT, Chen CH, Cheng TJ, Wu CY, Wong CH, 2010, “Differential receptor binding affinities of influenza hemagglutinins on glycan arrays.”, Journal of the American Chemical Society, 132(42), 14849-14856. (SCIE)
• Cheng TJ, Wu YT, Yang ST, Lo KH, Chen SK, Chen YH, Huang WI, Yuan CH, Guo CW, Huang LY, Chen KT, Shih HW, Cheng YS, Cheng WC, Wong CH, 2010, “High-throughput identification of antibacterials against methicillin-resistant Staphylococcus aureus (MRSA) and the transglycosylase.”, Bioorganic & medicinal chemistry, 18(24), 8512-8529. (SCIE)
• Su CY, Cheng TJ, Lin MI, Wang SY, Huang WI, Lin-Chu SY, Chen YH, Wu CY, Lai MM, Cheng WC, Wu YT, Tsai MD, Cheng YS, Wong CH, 2010, “High-throughput identification of compounds targeting influenza RNA-dependent RNA polymerase activity.”, Proceedings of the National Academy of Sciences of the United States of America, 107(45), 19151-19156. (SCIE)
• Cheng TJ, Sung MT, Liao HY, Chang YF, Chen CW, Huang CY, Chou LY, Wu YD, Chen YH, Cheng YS, Wong CH, Ma C, Cheng WC, 2008, “Domain requirement of moenomycin binding to bifunctional transglycosylases and development of high-throughput discovery of antibiotics.”, Proceedings of the National Academy of Sciences of the United States of America, 105(2), 431-436. (SCIE)